RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia

RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia

Blog Article

In hypoxic stress conditions, glycolysis enzymes assemble into singular cytoplasmic granules called glycolytic (G) bodies.G body formation in yeast correlates with increased glucose consumption and cell survival.However, the physical properties and organizing principles that define G body formation are unclear.

We demonstrate that glycolysis enzymes are non-canonical RNA binding proteins, sharing many common mRNA substrates that are also integral constituents of G bodies.Targeting nonspecific endoribonucleases to G bodies welding sweater reveals that RNA nucleates G body formation and maintains its structural integrity.Consistent with a phase separation mechanism of biogenesis, recruitment of glycolysis enzymes to G bodies relies on multivalent homotypic and heterotypic interactions.

Furthermore, G bodies fuse in vivo and are largely insensitive to 1,6-hexanediol, consistent with a hydrogel-like composition.Taken together, our results elucidate the biophysical nature of G 3m speedglas 9002nc bodies and demonstrate that RNA nucleates phase separation of the glycolysis machinery in response to hypoxic stress.